72/2026

1.1.

Beschaffer

Offizielle Bezeichnung: Aalborg Universitet

2.1.

Verfahren

Titel: Microbial single cell genomics analyses

Beschreibung: The Singe Cell Genomics Center (SCGC; https://scgc.bigelow.org) at the Bigelow Laboratory for Ocean Sciences is the only facility in the world capable of performing large-scale, integrated single-cell and single-viral-particle genome and phenome analyses on diverse environmental microbiomes. The processing of exceedingly small DNA quantities makes single cell genomics highly susceptible to DNA contamination and amplification biases. SCGC has developed techniques to monitor and minimize methodological artifacts at every step of its workflow. Cell sorting and DNA amplification are performed in a cleanroom, and all consumables are decontaminated using in-house methods. Control wells on each microplate are used to detect potential DNA contamination. To prevent index switching during DNA sequencing, multiplexed libraries contain dual 10 bp barcodes. Genome de novo assemblies are evaluated by diverse, in-house and external QC tools. The entire workflow is assessed for contamination and assembly errors using microbial benchmark cultures with diverse genome complexity and G+C content, indicating no non-target and undefined bases and average frequencies of mis-assemblies, indels, and mismatches at <5 per 100 kbp. The following SCGC services will be performed: 1. Generation of fluorescence-activated cell sorting (FACS) - based single amplified genomes (SAGs) (SCGC services S-201, S-202, and S-203). As part of these services, cells or other particles are separated by FACS, lysed, and their DNA is amplified in 384-well microplates. Cells/particles may be selected based on particle autofluorescence or fluorescent probes. Cells/particles are lysed and their DNA is denatured. Subsequently, the S-201 service uses WGA-X for genomic DNA amplification, a method developed by SCGC. Compared to the earlier versions of multiple displacement amplification technique (MDA), WGA-X improves average genome recovery from individual cells and viral particles, with most notable enhancements observed in SAGs with high G+C content. Service S-202 uses a genomic DNA amplification technique WGA-Y, which offers a further improvement in the average genome recovery from single cells. Service S-203 complements S-201 and S-202 by measuring oxygen respiration rates of individual microbial cells. Deliverables of FACS-based SAG generation services include: a. 384-well microplates containing WGA products of individual particles b. Phenotype measurements of sorted particles c. WGA kinetics in each well 2. Sequencing of FACS - based SAGs (services S-211, S-212, S-213, S-221, S-222, and S-223). These services include Illumina library preparation from SAG gDNA, shotgun sequencing, read demultiplexing, de novo assembly, functional and taxonomic annotation, and genome quality control. Deliverables include: a. raw sequence reads b. de novo SAG assemblies c. SAG functional annotation d. SAG taxonomic assignments e. general genome properties, such as GC content and coding density f. files to assist manual QC: outputs of tetramer PCA, BLASTn and checkM g. single particle reports. 3. Semi-permeable capsule (SPC) SAG generation and sequencing (services S-311, S-312, S-321, S-322, and S-323). Cells and other particles are compartmentalized into into SPCs using microfluidic platforms, then lysed, and their genomic DNA is amplified by WGA-Y to produce SPC SAGs. Environmental SPC SAGs are mixed with E. coli SPC SAGs, serving as internal standards. The SPC SAG DNA is combinatorially barcoded and then used in Illumina library preparation. After shotgun sequencing, reads are demultiplexed, de novo assembled, and the obtained assemblies are annotated and quality controlled. Deliverables include: h. barcoded SPC SAGs i. multiplexed Illumina libraries j. raw sequence reads k. de novo SAG assemblies l. SAG functional annotation m. SAG taxonomic assignments n. general genome properties, such as GC content and coding density o. files to assist manual QC 4. Complementary services: a. Handling of biohazard level 2 samples (S-004) b. FACS SAG re-arraying (S-005) c. Provision of cell cryoprotectant glyTE (S-009) d. Consultation (S-011) e. Customized services (S-020)

Kennung des Verfahrens: 4c391ebc-b4b8-456b-9a14-7297b705e275

Interne Kennung: 5860a0cb-6a24-4aff-a454-6d4e9ebb17b1

Verfahrensart: Verhandlungsverfahren ohne Aufruf zum Wettbewerb

2.1.1.

Zweck

Art des Auftrags: Dienstleistungen

Haupteinstufung (cpv): 73000000 Forschungs- und Entwicklungsdienste und zugehörige Beratung

Zusätzliche Einstufung (cpv): 73111000 Forschungslabordienste

2.1.2.

Erfüllungsort

Postanschrift: 60 Bigalow Drive East Boothbay

Stadt: Maine

Postleitzahl: 04544

Land: Vereinigte Staaten

2.1.4.

Allgemeine Informationen

Rechtsgrundlage:

Richtlinie 2014/24/EU

5.1.

Los: LOT-0000

Titel: Microbial single cell genomics analyses

Beschreibung: The Singe Cell Genomics Center (SCGC; https://scgc.bigelow.org) at the Bigelow Laboratory for Ocean Sciences is the only facility in the world capable of performing large-scale, integrated single-cell and single-viral-particle genome and phenome analyses on diverse environmental microbiomes. The processing of exceedingly small DNA quantities makes single cell genomics highly susceptible to DNA contamination and amplification biases. SCGC has developed techniques to monitor and minimize methodological artifacts at every step of its workflow. Cell sorting and DNA amplification are performed in a cleanroom, and all consumables are decontaminated using in-house methods. Control wells on each microplate are used to detect potential DNA contamination. To prevent index switching during DNA sequencing, multiplexed libraries contain dual 10 bp barcodes. Genome de novo assemblies are evaluated by diverse, in-house and external QC tools. The entire workflow is assessed for contamination and assembly errors using microbial benchmark cultures with diverse genome complexity and G+C content, indicating no non-target and undefined bases and average frequencies of mis-assemblies, indels, and mismatches at <5 per 100 kbp. The following SCGC services will be performed: 1. Generation of fluorescence-activated cell sorting (FACS) - based single amplified genomes (SAGs) (SCGC services S-201, S-202, and S-203). As part of these services, cells or other particles are separated by FACS, lysed, and their DNA is amplified in 384-well microplates. Cells/particles may be selected based on particle autofluorescence or fluorescent probes. Cells/particles are lysed and their DNA is denatured. Subsequently, the S-201 service uses WGA-X for genomic DNA amplification, a method developed by SCGC. Compared to the earlier versions of multiple displacement amplification technique (MDA), WGA-X improves average genome recovery from individual cells and viral particles, with most notable enhancements observed in SAGs with high G+C content. Service S-202 uses a genomic DNA amplification technique WGA-Y, which offers a further improvement in the average genome recovery from single cells. Service S-203 complements S-201 and S-202 by measuring oxygen respiration rates of individual microbial cells. Deliverables of FACS-based SAG generation services include: a. 384-well microplates containing WGA products of individual particles b. Phenotype measurements of sorted particles c. WGA kinetics in each well 2. Sequencing of FACS - based SAGs (services S-211, S-212, S-213, S-221, S-222, and S-223). These services include Illumina library preparation from SAG gDNA, shotgun sequencing, read demultiplexing, de novo assembly, functional and taxonomic annotation, and genome quality control. Deliverables include: a. raw sequence reads b. de novo SAG assemblies c. SAG functional annotation d. SAG taxonomic assignments e. general genome properties, such as GC content and coding density f. files to assist manual QC: outputs of tetramer PCA, BLASTn and checkM g. single particle reports. 3. Semi-permeable capsule (SPC) SAG generation and sequencing (services S-311, S-312, S-321, S-322, and S-323). Cells and other particles are compartmentalized into into SPCs using microfluidic platforms, then lysed, and their genomic DNA is amplified by WGA-Y to produce SPC SAGs. Environmental SPC SAGs are mixed with E. coli SPC SAGs, serving as internal standards. The SPC SAG DNA is combinatorially barcoded and then used in Illumina library preparation. After shotgun sequencing, reads are demultiplexed, de novo assembled, and the obtained assemblies are annotated and quality controlled. Deliverables include: h. barcoded SPC SAGs i. multiplexed Illumina libraries j. raw sequence reads k. de novo SAG assemblies l. SAG functional annotation m. SAG taxonomic assignments n. general genome properties, such as GC content and coding density o. files to assist manual QC 4. Complementary services: a. Handling of biohazard level 2 samples (S-004) b. FACS SAG re-arraying (S-005) c. Provision of cell cryoprotectant glyTE (S-009) d. Consultation (S-011) e. Customized services (S-020)

Interne Kennung: 21922a7d-b118-41c7-9160-ae48772bf4c8

5.1.1.

Zweck

Art des Auftrags: Dienstleistungen

Haupteinstufung (cpv): 73000000 Forschungs- und Entwicklungsdienste und zugehörige Beratung

Zusätzliche Einstufung (cpv): 73111000 Forschungslabordienste

5.1.2.

Erfüllungsort

Postanschrift: 60 Bigalow Drive East Boothbay

Stadt: Maine

Postleitzahl: 04544

Land: Vereinigte Staaten

5.1.6.

Allgemeine Informationen

Auftragsvergabeprojekt nicht aus EU-Mitteln finanziert

Die Beschaffung fällt unter das Übereinkommen über das öffentliche Beschaffungswesen: ja

5.1.15.

Techniken

Rahmenvereinbarung:

Keine Rahmenvereinbarung

Informationen über das dynamische Beschaffungssystem:

Kein dynamisches Beschaffungssystem

5.1.16.

Weitere Informationen, Schlichtung und Nachprüfung

Überprüfungsstelle: Klagenævnet for Udbud

Organisation, die zusätzliche Informationen über das Vergabeverfahren bereitstellt: Aalborg Universitet

Organisation, die weitere Informationen für die Nachprüfungsverfahren bereitstellt: Konkurrence- og Forbrugerstyrelsen

Organisation, aus deren Mitteln der Auftrag bezahlt wird: Aalborg Universitet

Direktvergabe:

Begründung der Direktvergabe: Der Auftrag kann nur von einem bestimmten Wirtschaftsteilnehmer ausgeführt werden, da aus technischen Gründen kein Wettbewerb vorhanden ist

Sonstige Begründung: The Singe Cell Genomics Center (SCGC; https://scgc.bigelow.org) at the Bigelow Laboratory for Ocean Sciences is the only facility in the world capable of performing large-scale, integrated single-cell and single-viral-particle genome and phenome analyses on diverse environmental microbiomes. The processing of exceedingly small DNA quantities makes single cell genomics highly susceptible to DNA contamination and amplification biases. SCGC has developed techniques to monitor and minimize methodological artifacts at every step of its workflow. Cell sorting and DNA amplification are performed in a cleanroom, and all consumables are decontaminated using in-house methods. Control wells on each microplate are used to detect potential DNA contamination. To prevent index switching during DNA sequencing, multiplexed libraries contain dual 10 bp barcodes. Genome de novo assemblies are evaluated by diverse, in-house and external QC tools. The entire workflow is assessed for contamination and assembly errors using microbial benchmark cultures with diverse genome complexity and G+C content, indicating no non-target and undefined bases and average frequencies of mis-assemblies, indels, and mismatches at <5 per 100 kbp. The following SCGC services will be performed: 1. Generation of fluorescence-activated cell sorting (FACS) - based single amplified genomes (SAGs) (SCGC services S-201, S-202, and S-203). As part of these services, cells or other particles are separated by FACS, lysed, and their DNA is amplified in 384-well microplates. Cells/particles may be selected based on particle autofluorescence or fluorescent probes. Cells/particles are lysed and their DNA is denatured. Subsequently, the S-201 service uses WGA-X for genomic DNA amplification, a method developed by SCGC. Compared to the earlier versions of multiple displacement amplification technique (MDA), WGA-X improves average genome recovery from individual cells and viral particles, with most notable enhancements observed in SAGs with high G+C content. Service S-202 uses a genomic DNA amplification technique WGA-Y, which offers a further improvement in the average genome recovery from single cells. Service S-203 complements S-201 and S-202 by measuring oxygen respiration rates of individual microbial cells. Deliverables of FACS-based SAG generation services include: a. 384-well microplates containing WGA products of individual particles b. Phenotype measurements of sorted particles c. WGA kinetics in each well 2. Sequencing of FACS - based SAGs (services S-211, S-212, S-213, S-221, S-222, and S-223). These services include Illumina library preparation from SAG gDNA, shotgun sequencing, read demultiplexing, de novo assembly, functional and taxonomic annotation, and genome quality control. Deliverables include: a. raw sequence reads b. de novo SAG assemblies c. SAG functional annotation d. SAG taxonomic assignments e. general genome properties, such as GC content and coding density f. files to assist manual QC: outputs of tetramer PCA, BLASTn and checkM g. single particle reports. 3. Semi-permeable capsule (SPC) SAG generation and sequencing (services S-311, S-312, S-321, S-322, and S-323). Cells and other particles are compartmentalized into into SPCs using microfluidic platforms, then lysed, and their genomic DNA is amplified by WGA-Y to produce SPC SAGs. Environmental SPC SAGs are mixed with E. coli SPC SAGs, serving as internal standards. The SPC SAG DNA is combinatorially barcoded and then used in Illumina library preparation. After shotgun sequencing, reads are demultiplexed, de novo assembled, and the obtained assemblies are annotated and quality controlled. Deliverables include: h. barcoded SPC SAGs i. multiplexed Illumina libraries j. raw sequence reads k. de novo SAG assemblies l. SAG functional annotation m. SAG taxonomic assignments n. general genome properties, such as GC content and coding density o. files to assist manual QC 4. Complementary services: a. Handling of biohazard level 2 samples (S-004) b. FACS SAG re-arraying (S-005) c. Provision of cell cryoprotectant glyTE (S-009) d. Consultation (S-011) e. Customized services (S-020)

6.1.

Ergebnis, Los-– Kennung: LOT-0000

6.1.2.

Informationen über die Gewinner

Wettbewerbsgewinner:

Offizielle Bezeichnung: Bigalow Laboratory for Ocean Sciences

Angebot:

Kennung des Angebots: Bigelow Laboratory for Ocean Sciences

Kennung des Loses oder der Gruppe von Losen: LOT-0000

Wert des Angebots: 5 000 000,00 DKK

Das Angebot wurde in die Rangfolge eingeordnet: nein

Informationen zum Auftrag:

Kennung des Auftrags: Microbial single cell genomics analyses

8.1.

ORG-0001

Offizielle Bezeichnung: Aalborg Universitet

Registrierungsnummer: 29102384

Postanschrift: Fredrik Bajers Vej 7K

Stadt: Aalborg Øst

Postleitzahl: 9220

Land, Gliederung (NUTS): Nordjylland (DK050)

Land: Dänemark

Kontaktperson: Alexander Herskind Rasmussen

E-Mail: ahra@adm.aau.dk

Telefon: 22505186

Rollen dieser Organisation:

Beschaffer

Organisation, die zusätzliche Informationen über das Vergabeverfahren bereitstellt

Organisation, aus deren Mitteln der Auftrag bezahlt wird

8.1.

ORG-0002

Offizielle Bezeichnung: Klagenævnet for Udbud

Registrierungsnummer: 37795526

Postanschrift: Toldboden 2

Stadt: Viborg

Postleitzahl: 8800

Land, Gliederung (NUTS): Vestjylland (DK041)

Land: Dänemark

Kontaktperson: Klagenævnet for Udbud

E-Mail: klfu@naevneneshus.dk

Telefon: +45 72405600

Internetadresse: https://naevneneshus.dk/start-din-klage/klagenaevnet-for-udbud/

Rollen dieser Organisation:

Überprüfungsstelle

8.1.

ORG-0003

Offizielle Bezeichnung: Konkurrence- og Forbrugerstyrelsen

Registrierungsnummer: 10294819

Postanschrift: Carl Jacobsens Vej 35

Stadt: Valby

Postleitzahl: 2500

Land, Gliederung (NUTS): Byen København (DK011)

Land: Dänemark

Kontaktperson: Konkurrence- og Forbrugerstyrelsen

E-Mail: kfst@kfst.dk

Telefon: +45 41715000

Internetadresse: https://www.kfst.dk

Rollen dieser Organisation:

Organisation, die weitere Informationen für die Nachprüfungsverfahren bereitstellt

8.1.

ORG-0004

Offizielle Bezeichnung: Bigalow Laboratory for Ocean Sciences

Postanschrift: 60 Bigalow Drive East Boothbay, Maine

Stadt: Maine

Postleitzahl: 04544

Land: Vereinigte Staaten

Rollen dieser Organisation:

Bieter

Gewinner dieser Lose: LOT-0000

8.1.

ORG-0005

Offizielle Bezeichnung: Mercell Holding ASA

Registrierungsnummer: 980921565

Postanschrift: Askekroken 11

Stadt: Oslo

Postleitzahl: 0277

Land, Gliederung (NUTS): Oslo (NO081)

Land: Norwegen

Kontaktperson: eSender

E-Mail: publication@mercell.com

Telefon: +47 21018800

Fax: +47 21018801

Internetadresse: http://mercell.com/

Rollen dieser Organisation:

TED eSender

Kennung/Fassung der Bekanntmachung: 458a364b-06a5-4d40-8d37-3f8f08ebc533 - 01

Formulartyp: Vorankündigung – Direktvergabe

Art der Bekanntmachung: Freiwillige Ex-ante-Transparenzbekanntmachung

Unterart der Bekanntmachung: 25

Datum der Übermittlung der Bekanntmachung: 13/04/2026 12:15:56 (UTC+00:00) Westeuropäische Zeit, GMT

Bekanntmachung — eSender-Übermittlungsdatum: 13/04/2026 12:21:03 (UTC+00:00) Westeuropäische Zeit, GMT

Sprachen, in denen diese Bekanntmachung offiziell verfügbar ist: Dänisch

Veröffentlichungsnummer der Bekanntmachung: 254240-2026

ABl. S – Nummer der Ausgabe: 72/2026

Datum der Veröffentlichung: 14/04/2026