High Resolution Masspectrometer (HRMS) — Q-Exactive Orbitrap LC-MS

The Department of Aquatic Sciences and Assessment has decided to invest in a High Resolution Mass Spectrometer (HRMS) of type Q-Exactive Orbitrap LC-MS to the laboratory of organic pollutants at the section of Organic Risk Pollutants Laboratory, (OMK)

Effect-Directed Analysis (EDA) requires utmost sensitivity, mass resolution and scanning speed. Because our focus is organic pollutants (and not large biomolecules) is the model we intend to acquire a Q-Exactive.

Huvudsaklig plats för byggnation/anläggning eller utförande: Sveriges Lantbruksuniversitet. Institutionen för Vatten och Miljö.

High resolution masspectrometer (HRMS) of typ Q-Exactive Orbitrap LC-MS.

Förklaring:

The Q-Exactive Focus is a benchtop quadrupole Orbitrap system based on the combination of Hyberbolic quadrupole technology combined with Orbitrap analyzer technique, designed for routine/small molecules applications. Implementation of a quadrupole mass filter in front of the curved ion trap (C-Trap) allows for precursor ion selection and hence for MS/MS and SIM scan modes besides the ability of full MS mode. PRM and SIM are also implemented in possible scan modes. In addition the high sensitivity RF-lens ion source, with the implementation of enhanced FT transient processing, provides scan speed and resolving power. Q-Exactive Focus is a very robust and very sensitive benchtop quadrupole high resolution mass spectromere perfectly combining precise quantification and high performance qualitative work in the instrument. This instrument is an Orbitrap-based FT-MS system providing high resolution, high mass accuracy and high sensitivity at an affordable price.

Mass range:

50 < m/z < 3000

Sensitivity:

Full MS: 500 fg Buspirone on column S/N 100:1

SIM: 50 fg Buspirone on column S/N 100:1

Resolution: at m/z 200 (scan rate)

17 500 (12 Hz)

35 000 (7 Hz)

70 000 (3 Hz)

Mass accuracy:

< 1 ppm RMS error with internal calibration

Quadrupole Isolation:

Stepless precursor isolation from 0.4 amu to full mass range

Scan modes:

Full Scan, MS/MS, SIM, SIM-MSX, PRM, AIF, vDIA (not for USA)

Polarity switching:

One full positive and negative scan cycle under 1 sec at a resolution

Setting of 35 000 (at m/z 200)

HCD fragmentation:

NCE

CE

The Q-Exactive Focus system consists of a quadrupole mass filter fully integrated with an Orbitrap Mass Spectrometer. Additionally included items:

— H-ESI II probe,

— Chemyx Fusion 100 Syringe pump with full data system control.

Visma annons: https://opic.com/id/afkolaavhy