Förklaring:

The Human Secretome project within the WCPR at KTH has since the start of the project (2013) been using a technology called the QMCF Technology (Patent number: EP1851319) developed by the Estonian company Icosagen Cell Factory OÜ. It is an expression system that uses specific mammalian cells (QMCF cell lines) and vectors (QMCF plasmids) for production of recombinant proteins and has been chosen after extensive testing of different expression systems. Today we have generated over 3 000 expression vectors that all are designed to work exclusively in Icosagen QMCF cells. Next step for our production pipeline is to produce higher amounts of each protein using these cells and vectors. During the last year one of the researchers in the Protein Factory group has evaluated different protocols. For example, different culture media, feed media, shake flasks and transfection reagents have been evaluated for the upscaled production. The optimized protocol now choosen for maintaining a high transfected cell density, viability, and to achieve the highest titers in the Icosagen system includes media produced and distributed by Irvine Scientific. This new cultivation protocol also works in a high-throughput way that is necessary for our production pipeline.

Because of the above stated reasons it is crucial for our upscaled production that we are able to buy this culture medium and feed medium from Irvine Scientific that are the manufacturer of these media and the only company that sells these products.