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Denmark-Copenhagen: Laboratory, optical and precision equipments (excl. glasses)
Voluntary ex ante transparency notice
Section I: Contracting authority/entity
Section II: Object
Acquisition of an Automated Liquid Handling System Based on the Hamilton Microlab STAR Line
The proteomics research infrastructure (PRI) would like to purchase an automated liquid handling system based on the Hamilton Microlab STAR line.
The proteomics research infrastructure (PRI) would like to purchase an automated liquid handling system based on the Hamilton Microlab STAR line for the purpose of streamlined, robust and high-throughput preparation of user samples.
Section IV: Procedure
An automatic liquid handling platform is required for the preparation of user samples in a high-throughput manner. The system needs to be equipped with an on-deck solid phase extraction (SPE) device, centrifuge, an eight-channel pipetting head with individually moving channels, a 96-channel pipetting head with liquid sensing and pressure detection of individual channels and a robotic arm to move between deck and side positions.
It further needs to integrate following devices: on-deck thermocyclers for 96 and 384 well plates, screw cap tube opener in 96 well format, Thermo Kingfisher device for magnetic bead based enrichment protocols, temperature controlled microplate shaker with a temperature range for incubation of microplate and deep-well plates at 4 ºC to 70 ºC, incubator shaker, 2D tube bottom barcode reader, and a 1D plate barcode reader.
The PRI has three mass spectrometers (MS), running in 24/7 mode from the first quartal of 2021 with an estimated increase to six MS systems in mid 2022. While MS analyses are highly streamlined, sample preparation is the clear bottleneck in the pipeline from sample submission to providing results. A robust and scalable automated sample preparation pipeline can solve this need.
The Hamilton Microlab STARline is the only system on the market, which meets the technical requirement for automating all of our sample preparation protocols. The system comes with an on-deck positive pressure module for desalting and buffer exchange purposes ([MPE]2). Its unique design allows direct loading of buffers and samples onto solid phase extraction plates using the 96-channel pipetting head. Compared to off-deck solutions this increases throughput as well as reduces buffer consumption given that all loading steps are carried out by the pipetting head, instead of a pump.
Furthermore, the Microlab STAR line is the only liquid handling system that can handle volumes from 1 to 1 000 l with a single 96-channel pipetting head that covers the complete volume range used in our protocols without the need of head exchange.
An additional unique feature of the STAR 96-channel head is its liquid level sensing and pressure detection capability on all channels. While liquid level sensing can detect empty wells, pressure sensing monitors effective pipetting and therefore detects cases in which samples are not correctly transferred, for instance due to excessive viscosity. In both cases the software flags the respective samples providing an efficient way of error control and a handle for manual intervention.
Total aspiration and dispense monitoring (TADM):
TADM for 96-channel Multi-Probe Head:
Anti droplet control (ADC):
Section V: Award of contract/concession
Section VI: Complementary information